Fig 1: PAI-1 expression is increased in bladder cancer. Representative images of normal urothelium (A) and of bladder cancers with absent (B), weak (C) and strong (D) PAI-1 expression (brown) are shown. Representative images of a sample TMA core (E), PAI-1 expression in the tumor-associated stroma (F) and PAI-1 expression in the tumor epithelia (G). Scale bars, 100 µm. (H) Quantification of PAI-1 expression levels in low-grade (LG) vs. high-grade (HG) and NMIBC vs. MIBC. (I) Correlation between PAI-1 expression and overall survival in NMIBC and MIBC. Data are from analysis of a TMA containing 939 bladder tumors. (J) PAI-1 expression is noted in the nucleus and cytoplasm.
Fig 2: PAI-1 subcellular localization and ChIP-seq profiling. (A) Depicted is the cell fractionation protocol for isolating cytoplasm and nuclear protein. (B) All fractions including whole cell lysate (WCL), nuclear (Nuc), or cytoplasmic extracts (Cyto) from UM-UC-3 and RT112 cells were evaluated by immunoblot analysis. (C) Subcellular localization of PAI-1 (red) was visualized using specific antibody. (D) Histogram demonstrating distribution of PAI-1 binding across the genome in UM-UC-3 cells. The frequency of PAI-1 binding across chromosomes was calculated by dividing the number of probe sets per chromosome by the number of probe sets bound by PAI-1 with FDR < 0.2. (E) Histogram demonstrating relative PAI-1 bound peak location with respect to chromosome region in UM-UC-3 cells. (F) Pie chart of the genomic location distribution of PAI-1 in UM-UC-3 cells. This plot shows the percentage for each genomic location category. The categories are sorted by descending percentage.
Fig 3: Representative expression status for ANG, MMP-2, p53, RB and PAI-1 in a high-grade non-muscle invasive bladder cancerUpper row represents high intensity while lower row represents low intensity associated with each target. All images were captured at 400× magnification.
Fig 4: Gene expression profiling by RNA-seq and integration of ChIP-seq and RNA-seq. (A) Enrichment Analysis of RNA-seq indicate that knockdown of PAI-1 results in upregulation of the genes. (B) Volcano plot showing fold-change and p-value for the comparisons of negative control and PAI-1 siRNA. (C) Venn diagram showing the overlapping genes between ChIP-seq and upregulated genes from RNA-seq. (D) Venn diagram representing the overlap of nuclear PAI-1 regulating genes in UM-UC-3 and RT112 cells.
Fig 5: Overall survival analysis of 939 patients with bladder cancerOverall survival according to immunostaining status of ANG, MMP-2, p53, RB and PAI-1 in NMIBC (A) and MIBC (B).
Supplier Page from MilliporeSigma for Anti-SERPINE1 antibody produced in rabbit